Table 8

HAD	Substrate	pH	Metal	Km	Vmax	k_cat	k_cat/K_m	H
				mM	mmol/min x mg	s^-1	M^-1s^-1
SC1 (Ydl236W)
	P-Glycolate	7.5	Mg⁺²	0.061 ± 0.003	116.7 ± 1.3	67.35	1.10E+06
	P-Glycolate	7.5	Co⁺²	0.098 ± 0.005	148.6 ± 1.7	85.75	8.73E+05
SC2 (YEL038W)
	P-Glycolate	7.2	Mg⁺²	0.07 ± 0.01	0.243 ± 0.009	0.11	1.62E+03
	P-Glycolate	7.0	Mn⁺²	0.051 ± 0.004	0.088 ± 0.001	0.041	8.04E+02
	P-Glycolate	7.0	Co⁺²	0.044± 0.003	0.177 ± 0.003	0.083	1.90E+03

	P-Glycolate	7.0	Mg⁺²	0.0401 ± 0.004	0.178 ± 0.004	0.08	2.08E+03
SC3 (YER037W)
	CMP	6.5	Mg⁺²	0.063 ± 0.002	29.62 ± 0.21	19.79	3.16E+05
	CMP	6.5	Co⁺²	0.036 ± 0.002	4.76 ± 0.05	3.18	8.77E+04
	CMP	6.5	Ni⁺²	0.095 ± 0.002	14.59 ± 0.06	9.75	1.02E+05

	XMP	7.0	Mg⁺²	0.52 ± 0.02	42.81 ± 0.94	28.61	5.51E+04	1.44 ± 0.09
	XMP	7.0	Mn⁺²	0.32 ± 0.02	19.55 ± 0.29	13.07	4.07E+04
	XMP	7.0	Co⁺²	0.39 ± 0.02	9.1 ± 0.2	6.1	1.57E+04	1.79 ± 0.19
SC4 (YER062C)
	Glycerol-3P	6.5	Mg⁺²	3.02 ± 0.12	239.2 ± 2.8	122.27	4.04E+04
	Glycerol-3P	6.5	Mn⁺²	0.41 ± 0.02	22.89 ± 0.24	11.7	2.82E+04
	Glycerol-3P	6.5	Co⁺²	2.39 ± 0.14	140.6 ± 2.6	71.88	3.01E+04

	Glycerol-1P	6.5	Mg⁺²	4.72 ± 0.15	222.6 ± 2.2	113.78	2.41E+04
	Glycerol-1P	6.5	Mn⁺²	0.52 ± 0.01	21.77 ± 0.11	11.13	2.14E+04
	Glycerol-1P	6.5	Co⁺²	2.83 ± 0.09	114.8 ± 1.03	58.68	2.07E+04
SC5 (Ygl224c)
	UMP*	7.0	Mg⁺²	0.046 ± 0.003	6.38 ± 0.09	3.66	7.90E+04
	UMP*	7.0	Mn⁺²	0.013 ± 0.001	1.97 ± 0.02	1.13	8.77E+04
	UMP	7.0	Co⁺²	0.22 ± 0.01	2.76 ± 0.02	1.59	7.28E+03

	CMP*	7.0	Mg⁺²	0.11 ± 0.01	9.75 ± 0.08	5.59	4.97E+04
	CMP*	7.0	Mn⁺²	0.035 ± 0.001	2.44 ± 0.01	1.4	3.98E+04
	CMP	7.0	Co⁺²	0.33 ± 0.03	3.75 ± 0.09	2.15	6.54E+03
SC6 (Ygr208w)
	P-Serine	7.0	Mg⁺²	0.08 ± 0.01	205.9 ± 6.9	117.4	1.41E+06
	P-Serine*	7.0	Mg⁺²	0.06 ± 0.01	171.4 ± 7.8	97.7	1.60E+06
	P-Serine	7.0	Co⁺²	0.13 ± 0.01	127.4 ± 3.9	72.63	5.62E+05
SC7 (YHR043C)
	2d-Glucose-6P	6.75	Mg⁺²	0.769 ± 0.038	33.92 ± 0.95	16.97 ± 0.47	2.20E+04	1.55 ± 0.07
	2d-Glucose-6P	6.75	Mn⁺²	0.62 ± 0.03	5.98 ± 0.12	2.99 ± 0.06	4.82E+03	1.35 ± 0.05

	Mannose-6P	6.75	Mg⁺²	1.05 ± 0.06	27.48 ± 0.93	13.75 ± 0.46	1.31E+04	1.36 ± 0.05
	Mannose-6P	6.75	Mn⁺²	0.99 ± 0.08	5.88 ± 0.26	2.94 ± 0.13	2.94E+03	1.31 ± 0.06

	Fructose-1P	7.0	Mg⁺²	1.77 ± 0.08	57.95 ± 0.94	28.99	1.64E+04
	Fructose-1P	7.0	Mn⁺²	0.58 ± 0.01	7.81 ± 0.06	3.91	6.77E+03
SC8 (YHR044C)
	2d-Glucose-6P	6.75	Mg⁺²	1.39 ± 0.07	199.4 ± 4.2	99.55	7.12E+04	1.14 ± 0.04
	2d-Glucose-6P	6.75	Mn⁺²	0.53 ± 0.02	51.75 ± 0.68	25.84	4.84E+04	1.16 ± 0.02

	Mannose-6P	7.0	Mg⁺²	5.72 ± 0.14	92.86 ± 1.15	46.36	8.10E+03
	Mannose-6P	7.0	Mn⁺²	2.55 ± 0.09	14.5 ± 0.2	7.22	2.84E+03
SC9 (YIL053W)
	Glycerol-3P	6.5	Mg⁺²	3.61 ± 0.11	199.7 ± 1.8	102.55	2.84E+04
	Glycerol-3P*	6.5	Mg⁺²	3.29 ± 0.09	227.2 ± 1.8	116.67	3.55E+04
	Glycerol-3P*	6.5	Mn⁺²	0.49 ± 0.03	26.1 ± 0.4	13.4	2.68E+04
	Glycerol-3P	6.5	Co⁺²	3.01 ± 0.06	93.4 ± 0.6	47.95	1.60E+04

	Glycerol-1P	6.5	Mg⁺²	2.95 ± 0.46	151.3 ± 6.6	77.68	2.63E+04
	Glycerol-1P	6.5	Mn⁺²	2.12 ± 0.18	23.9 ± 0.6	12.31	5.81E+03
SC10 (YKL033W-A)
	XMP	7.2	Mg⁺²	1.26 ± 0.06	3.97 ± 0.09	1.92	1.53E+03	1.21 ± 0.03
	XMP	7.2	Mn⁺²	1.11 ± 0.03	1.61 ± 0.02	0.78	7.02E+02
	XMP	7.5	Co⁺²	1.34 ± 0.05	2.32 ± 0.05	1.12	8.42E+02	1.94 ± 0.07

	FMN	7.2	Mg⁺²	0.19 ± 0.01	0.33 ± 0.01	0.16	8.18E+02
	FMN	7.5	Mn⁺²	0.06 ± 0.01	0.13 ± 0.01	0.063	1.01E+03
	FMN	7.5	Co⁺²	0.089 ± 0.007	0.197 ± 0.003	0.095	1.06E+03
SC11 (YKR070W)
	Glucose-6P	7.0	Mg⁺²	0.25 ± 0.02	41.98 ± 0.98	29.57	1.19E+05
	Glucose-6P*	7.0	Mg⁺²	0.22 ± 0.02	49.81 ± 1.13	35.08	1.62E+05
	Glucose-6P	7.0	Mn⁺²	0.058 ± 0.005	11.26 ± 0.18	7.93	1.37E+05
	Glucose-6P*	7.0	Mn⁺²	0.076 ± 0.009	9.94 ± 0.28	7.00	9.24E+04
	Glucose-6P	7.0	Co⁺²	0.17 ± 0.02	25.91 ± 0.64	18.25	1.06E+05

	Glycerol-3P*	7.0	Mg⁺²	0.28 ± 0.02	62.63 ± 1.14	44.11	1.56E+05
	Glycerol-3P*	7.0	Mn⁺²	0.11 ± 0.01	33.24 ± 0.49	23.41	2.04E+05
	Glycerol-3P	7.0	Co⁺²	0.31 ± 0.02	54.85 ± 0.82	38.63	1.25E+05

	Glycerol-1P	7.0	Mg⁺²	0.39 ± 0.05	50.51 ± 1.83	35.58	9.15E+04
	Glycerol-1P	7.0	Mn⁺²	0.15 ± 0.01	26.42 ± 0.45	18.61	1.23E+05
	Glycerol-1P	7.0	Co⁺²	0.35 ± 0.02	43.1 ± 0.8	30.29	8.78E+04

	2d-Glucose-6P	7.0	Mg⁺²	0.59 ± 0.03	65.63 ± 0.84	46.23	7.75E+04
	2d-Glucose-6P	7.0	Mn⁺²	0.13 ± 0.01	20.43 ± 0.31	14.39	1.08E+05
	2d-Glucose-6P	7.0	Co⁺²	0.41 ± 0.03	52.73 ± 0.89	37.14	8.97E+04

	6P-Gluconate	7.0	Mg⁺²	0.45 ± 0.02	46.84 ± 0.63	32.99	7.38E+04
	6P-Gluconate	7.0	Mn⁺²	0.16 ± 0.01	3.81 ± 0.04	2.69	1.72E+04
	6P-Gluconate	7.0	Co⁺²	0.36 ± 0.02	41.31 ± 0.62	29.09	8.12E+04

	2d-Ribose-5P	7.0	Mg⁺²	0.31 ± 0.01	38.71 ± 0.55	27.27	8.76E+04	1.46 ± 0.06
	2d-Ribose-5P*	7.0	Mg⁺²	0.44 ± 0.03	40.35 ± 0.92	28.42	6.44E+04
	2d-Ribose-5P	7.0	Mn⁺²	0.12 ± 0.01	11.11 ± 0.31	7.83	6.45E+04
	2d-Ribose-5P	7.0	Co⁺²	0.38 ± 0.01	38.8 ± 0.6	27.33	7.28E+04	1.47 ± 0.06

	Fructose-1,6 bisP*	7.0	Mg⁺²	0.65 ± 0.02	56.87 ± 0.48	40.06	6.20E+04
	Fructose-1,6 bisP	7.0	Mn⁺²	0.43 ± 0.02	17.93 ± 0.17	12.63	2.94E+04

	Fructose-6P*	7.0	Mg⁺²	1.04 ± 0.05	95.71 ± 0.84	67.41	6.46E+04
	Fructose-6P*	7.0	Mn⁺²	0.76 ± 0.01	26.23 ± 0.15	18.48	2.45E+04
	Fructose-6P	7.0	Co⁺²	0.53 ± 0.03	32.58 ± 0.59	22.95	4.34E+04

	Erythrose-4P	7.0	Mg⁺²	0.33 ± 0.07	41.9 ± 2.2	29.53	9.00E+04
	Erythrose-4P	7.0	Mn⁺²	0.28 ± 0.02	14.98 ± 0.21	10.55	3.77E+04

	Mannose-6P	7.0	Mg⁺²	0.65 ± 0.06	48.7 ± 1.3	34.27	5.31E+04
	Mannose-6P*	7.0	Mg⁺²	0.67 ± 0.03	56.27 ± 0.85	39.63	5.93E+04
	Mannose-6P*	7.0	Mn⁺²	0.21 ± 0.01	16.98 ± 0.23	11.96	5.67E+04
	Mannose-6P	7.0	Co⁺²	0.53 ± 0.03	34.07 ± 0.55	23.99	4.54E+04

	Ribose-5P*	7.0	Mg⁺²	0.75 ± 0.03	69.61 ± 0.52	49.03	6.51E+04
	Ribose-5P*	7.0	Mn⁺²	0.25 ± 0.01	17.95 ± 0.14	12.64	5.12E+04

	Glycerol-2P	7.0	Mg⁺²	1.22 ± 0.04	17.25 ± 0.20	12.15	9.95E+03
	Glycerol-2P	7.0	Mn⁺²	0.37 ± 0.03	2.85 ± 0.05	2.01	5.45E+03
	Glycerol-2P	7.0	Co⁺²	1.75 ± 0.08	18.89 ± 0.29	13.31	7.62E+03
SC13 (YNL010W)
	Glycerol-1P*	6.75	Mg⁺²	1.12 ± 0.06	70.78 ± 1.02	35.79	3.19E+04
	Glycerol-1P*	7.0	Mn⁺²	0.72 ± 0.04	11.48 ± 0.14	5.8	8.05E+03
	Glycerol-1P	7.2	Co⁺²	2.81 ± 0.21	38.55 ± 0.86	19.49	6.93E+03

	Erythrose-4P	6.75	Mg⁺²	2.84 ± 0.11	51.94 ± 0.78	26.26	9.26E+03
	Erythrose-4P	7.0	Mn⁺²	1.78 ± 0.14	10.05 ± 0.25	5.08	2.86E+03
SC14 (YOR131C)
	PLP	7.0	Mg⁺²	0.071 ± 0.004	6.48 ± 0.09	2.99	4.23E+04
	PLP*	7.0	Mg⁺²	0.35 ± 0.01	4.91 ± 0.07	2.27	6.41E+03
	PLP	6.75	Mn⁺²	0.019 ± 0.002	0.68 ± 0.01	0.31	1.66E+04
	PLP	7.0	Co⁺²	0.13 ± 0.01	2.78 ± 0.05	1.29	9.98E+03

	Imido-diP	7.0	Mg⁺²	0.91 ± 0.12	5.71 ± 0.34	2.64	2.90E+03	1.49 ± 0.21
	Imido-diP	7.0	Mg⁺²	0.69 ± 0.05	6.56 ± 0.23	3.03	4.38E+03	1.34 ± 0.09
	Imido-diP	7.0	Co⁺²	0.79 ± 0.09	3.27 ± 0.17	1.51	1.92E+03	1.45 ± 0.19

* DTT used in reaction mixture at 0.06 mM

Yeast Integrative Biology Project

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